Biolog ECO板及培養(yǎng)基優(yōu)勢促銷 整箱起訂 ，量多更優(yōu)惠 詳情請來電 15810200961
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美國BIOLOG公司獨家研發(fā)的產(chǎn)品 ECO板，特別為微生物群落分析和生態(tài)研究設(shè)計，Eco板上包含國際土壤微生物學(xué)家精選的用于微生物群落分析最常用的31種碳源，是國際上微生物生態(tài)功能多樣性研究的最典型和最權(quán)威方法之一。
每塊96孔ECO板上有3組平行，每組31種碳源，3個陰性對照，加入同一樣品后，可以獲得3組平行數(shù)據(jù)。微生物群落對這31種碳源的特征性利用稱作該微生物群落的代謝指紋圖譜。從單個的微平板上的指紋圖譜就可以獲得大量的代謝信息?？捎糜诩兎N及混合菌群(如土壤樣品)微生物功能多樣性研究。
31種碳源可分為六大類，具體如下：
單糖\糖苷\聚合糖類
B2， D-木糖
H1, a-D-乳糖
A2, ß-甲基D-葡萄糖苷
G2,葡萄糖-1-磷酸鹽
E1, a-環(huán)狀糊精
F1, 肝糖
G1, D-纖維二糖
氨基酸類，
A4，L-精氨酸，
B4，L-天冬酰胺酸
C4 L-苯基丙氨酸
D4, L-絲氨酸
E4，L-蘇氨酸
F4，甘氨酰-L-谷氨酸
酯類
B1, 丙酮酸甲酯
C1, 吐溫40
D1, 吐溫80
A3, D-半乳糖酸γ內(nèi)酯
醇類
C2, I-赤藻糖醇
D2, D-甘露醇
H2, D,L-a-甘油
胺類
G4, 苯乙基胺
H4, 腐胺
E2, N-乙酰基-D-葡萄胺
酸類
B3,D-半乳糖醛酸
F2 D-氨基葡萄糖酸
C3, 2-羥苯甲酸
D3, 4-羥基苯甲酸
E3,r-羥基丁酸
F3, 衣康酸
G3,a-丁酮酸
H3, D-蘋果酸
樣品簡單處理后(過濾或離心)，取上清液加到ECO板上培養(yǎng)多至5天時間，每隔一定時間用酶標(biāo)儀在590nm和750nm下測定OD值，OD值的大小反應(yīng)樣品微生物利用某一碳源底物的能力，將OD值導(dǎo)出后進行分析，如AWCD值(平均吸光度)、豐富度、單孔的動力學(xué)分析、不同類碳源的比較等，并可用聚類軟件對不同樣品進行聚類分析。
值得一提的是ECO板不僅測定可培養(yǎng)微生物，還可測定部分不可培養(yǎng)微生物，只要微生物能利用碳源進行代謝(并不增殖)，ECO板上顯色體系均可檢測出來。
最常見的應(yīng)用是利用ECO板分析環(huán)境因素、根系、葉面、肥料、農(nóng)藥對土壤微生物菌群的生態(tài)功能多樣性的影響，比較不同樣品間或同一樣品不同時間的功能多樣性差異，比較環(huán)境修復(fù)前后微生物群落的功能多樣性差異，等等。
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BIOLOG鑒定板與培養(yǎng)基
Microplate Panels and Media
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GEN II & GEN III MicroPlate Consumables
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· Sold in increments of 10 plates/box
· Consumable products are NON-RETURNABLE
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Catalog Number
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Description
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1030
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GEN III MicroPlate (for both Gram-negative & Gram-positive bacteria)
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1011
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GN2 MicroPlate (GEN II for Gram-negative bacteria)
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1014
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GP2 MicroPlate (GEN II for Gram-positive bacteria)
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1007
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AN MicroPlate (GEN II for Anaerobic bacteria)
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1005
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YT MicroPlate (GEN II for Yeasts)
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1006
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FF MicroPlate (GEN II for Filamentous Fungi)
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Special Purpose MicroPlates
· Sold in increments of 10 plates/box
· Consumable products are NON-RETURNABLE
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Catalog Number
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Description
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1013
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MT2 MicroPlate (for metabolic studies)
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1511
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SF-N2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-negative microbes)
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1514
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SF-P2 MicroPlate (for metabolic characterization of spore-forming and conidia-forming gram-positive microbes)
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1506
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ECO MicroPlate - 3 sets of the same 31 carbon sources in a 96 well Biolog MicroPlate. Used for characterization / community analysis)
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Biolog Prepared Plated Media
· Sold in increments of 15 plates/pack
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Catalog Number
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Description
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71102
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BUG (Biolog Universal Growth) Agar w/ 5% sheep blood
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71103
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BUG (Biolog Universal Growth) Agar w/ 0.25% maltose1
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71005
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BUY (Biolog Universal Yeast) Agar1
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Other Prepared Plated Media
· Sold in increments of 10 plates/pack
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Catalog Number
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Description
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Bio-M1040
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Tryptic Soy Agar (TSA)
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Bio-M1006
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Tryptic Soy Agar (TSA) w/ 5% sheep blood
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Bio-M1012
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Chocolate Agar, enriched
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71007
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BUA (Biolog Universal Anaerobe) Agar w/5% sheep blood
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Bio-M1065
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R2A Agar
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71106
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2% Malt Extract Agar
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Biolog Dehydrated Media
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Catalog Number
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Description
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70101
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BUG (Biolog Universal Growth) Agar - 500 g jar (approx. 435 plates)
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70007
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BUA (Biolog Universal Anaerobe) Agar
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70005
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BUY (Biolog Universal Yeast) Agar - 500 g jar (approx. 415 plates)
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GEN III Starter Kits
· Kits containing the consumables a new user would need to begin using the OmniLog System for bacterial indentifications
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Catalog Number
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Description
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39993A-O
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20 GEN III MicroPlates
· 1 box of pipette tips (Ovation)
· 1 CASE OF reservoirs
· 1 package OF Inoculatorz (100 units individually packaged)
· 20 tubes OF IF-A
· 20 tubes OF IF-B
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39993A-M
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20 GEN III MicroPlates
· 1 box of pipette tips (Matrix)
· 1 CASE OF reservoirs
· 1 package OF Inoculatorz (100 units individually packaged)
· 20 tubes OF IF-A
· 20 tubes OF IF-B
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39993B
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30 BUG+blood plated media for GEN III
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