北京智杰方遠(yuǎn)代理臺(tái)灣旭基――病毒核酸純化試劑盒VR100現(xiàn)貨促銷

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Virus DNA/RNA Extraction Kit II VR050/VR100/VR300

The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 10¹-10⁹ copies of viral DNA/RNA can be purified from up to 200 µl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.

Advantages (Cat. # VR050, VR100, VR300)

· High Sensitivity: virus RNA/DNA can be successfully extrac　　　　　　　　　　　　　　　　　　　　　   ted and detected from as low as 10E1 copy number!

· Purify virus DNA or virus RNA in 20 minutes!

· Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures

· Spin Columns: glass fiber membrane optimized for virus DNA and virus RNA purification

· Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free

· Elution Volume: 50 µl

· Storage: dry at room temperature (15-25ºC)

Applications

RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS) 

Components

· VB Lysis Buffer

· AD Buffer

· W1 Buffer

· Wash Buffer

· RNase-free Water

· VB Columns

· 2 ml Collection Tubes

Quality Control

The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaids ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.

Viral Nucleic Acid Extraction Kit II Functional Test Data

Figure 1. Virus RNA was purified from 10E1-10E4 copy number of Red Spotted Grouper Nervous Necrosis Virus (RGNNV) using the Geneaid Virus DNA/RNA Kit II (3 replications of each copy number). The purified RNA was eluted with 30 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 μl. A Real-time PCR assay was then performed with 3 μl of synthesized cDNA as template, primers (designed to amplify the T4 region on the RNA2 segment), and Fast SYBR Green PCR Master Mix using the StepOnePlusTM Real-Time PCR system (Applied Biosystems). The results confirmed that virus RNA can be successfully extracted and detected from as low as 10E1 copy number of RGNNV. The average cycle threshold (Ct): 10E4 = 23.88, 10E3 = 27.72, 10E2 = 31.22, 10E1 = 34.62. The low Ct values indicate a high number of target nucleic acid in the sample.

Figure 3. HBV (DNA), HCV (RNA), HIV (RNA), and HTLV (RNA) were purified from 200 µl of positive clinical serum samples using the Viral Nucleic Acid Extraction Kit II. Real-time qPCR and 1-step qRT-PCR reactions were then conducted using the ABI 7300 Sequence Detection System (3 replications of each copy number). Serum samples containing various amounts of DNA/RNA viruses ranging from 10E1 to 10E6 copies/ml were successfully detected and identified. The low Ct values indicate a high number of target nucleic acid in the sample.

Publications

Journal Article 1

Journal Article 2

Journal Article 3

Journal Article 4

Journal Article 5

Related Virus DNA/RNA Purification Products

病毒基因組DNA/RNA 提取試劑盒

僅供科研使用

產(chǎn)品編號(hào)

VR050, VR100, VR300

完整說明書(英文)下載

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1. 處理材料

取200 μl樣本（例如血漿,血清,淋巴液或經(jīng)病毒感染的細(xì)胞培養(yǎng)液）轉(zhuǎn)移到1.5 ml微量離心管中.

注意：樣本不足200 μl可加緩沖液PBS補(bǔ)足.加入400 μl緩沖液VB Lysis,使用渦旋振蕩器振蕩混勻,室溫放置10分鐘.

2. 吸附

加入450 μl緩沖液AD（使用前請(qǐng)先檢查是否已加入無水乙醇),充分搖動(dòng)混勻. 將吸附柱VB放入2 ml收集管中. 將600 μl所得溶液加入吸附柱VB中,以14-16,000×g離心1分鐘,倒掉收集管中的廢液,將吸附柱放回收集管中. 將剩余的溶液加入吸附柱VB中,以14-16,000×g離心1分鐘,丟棄收集管,將吸附柱放入新的收集管中.

3. 漂洗

向吸附柱VB中加入400 μl漂洗液W1,以14-16,000 ×g離心30秒,倒掉收集管中的廢液,將吸附柱放入收集管中. 向吸附柱VB中加入600 μl漂洗液Wash（使用前請(qǐng)先檢查是否已加入無水乙醇),以14-16,000×g離心30秒,倒掉收集管中的廢液,將吸附柱VB放入收集管中. 以14-16,000 ×g離心3分鐘將吸附柱中殘余的漂洗液去除.

注意:漂洗液中乙醇的殘留會(huì)影響后續(xù)實(shí)驗(yàn).

4. 洗脫

將吸附柱VB放入一個(gè)RNase-free的離心管中,向吸附膜中間位置懸空滴加50 μl洗脫緩沖液RNase-freewater,室溫放置3分鐘后,以14-16,000 ×g離心1分鐘收集病毒DNA/RNA溶液。